human dermal microvascular endothelial cells cell line  (PromoCell)

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Effect of OA on viability and LDH release in human (HMEC-1) and mouse (MS1) microvascular endothelial cells. a Cell viability of HMEC-1 cells treated with OA for 3, 6–24 h. b Cell viability of MS1 cells treated with OA for 3, 6–24 h. Results are expressed as percentage of control cells and presented as mean ± SEM of three independent replicates performed by triplicate. c LDH release in HMEC-1 cells and d) MS1 cells treated with OA for 3, 6–24 h. Data are expressed as percentage of cell death control and presented as mean ± SEM of three independent replicates performed by duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to untreated control).

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Control

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Measurement of IL-6 and CD147 release from human (HMEC-1) and mouse (MS1) microvascular endothelial cells treated with OA. a IL-6 release in HMEC-1 and b IL-6 release in MS1 after OA treatment. c Levels of CD147 receptor measured in HMEC-1. d Levels of CD147 receptor measured in MS1. LPS at 500 ng/ml was used as a positive control. Mean ± SEM of three independent replicates performed by duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001 **** p < 0.0001 compared to untreated control.

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Positive Control, Control

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Analysis of NFκB and STAT3 activation in human (HMEC-1) and mouse (MS1) microvascular endothelial cells after treatment with OA. a Activation of NFκB-p65 in HMEC-1 cells and b activation of NFκB-p65 in MS1 cells. c Activation of STAT3 in HMEC-1 and d activation of STAT3 in MS1 cells. Data are present as ratio of phosphorylated and total protein and normalized by actin. Cells were incubated with 100 nM OA for 3 and 6 h. LPS 500 ng/mL was used as positive control. Mean ± SEM from three independent replicates performed in duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to untreated control).

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Activation Assay, Incubation, Positive Control, Control

Journal: bioRxiv

Article Title: A Novel Humanized Immune Stroma PDX Cancer Model for Therapeutic Studies

doi: 10.1101/2023.07.03.547206

Figure Lengend Snippet: ( a ) Tumor growth curves (i – iii) and (iv) summary of relative final tumor volumes of HS-PDXs with the indicate EC/EPC sources. TC + CA-MSC in the absence or presence of human EC (TC + CA-MSC; n = 6) (iHDMEC + iHUVEC) and Pt. EC/EPC (TC + CA-MSC + EC/EPC; n = 7). ( b ) IF evaluation of human tumor vascular antigen CD31 (red) in PDXs with the indicated EC/EPC sources. 4′,6-diamidino-2-phenylindole (DAPI; blue) is used to label cell nuclei. ( c ) IF evaluation of a tumor specific vascular marker EGFL6 (red). Cell nuclei were counterstained with DAPI (blue). ( d ) RNASeq based relative mRNA expression for two patient PDXs without and with the addition of human endothelial cells. (e) Growth curves for standard PDX and HS-PDX, derived from a patient with platinum refractory ovarian cancer, treated with carboplatin (blue arrows indicate timing of carboplatin administration. Abbreviations : CA-MSC, cancer-associated mesenchymal stem cells; EC, endothelial cells; EPC, endothelial progenitor cells; iHDMEC, immortalized human dermal microvascular endothelial cells; iHUVEC, immortalized human umbilical vein endothelial cells; PDX, patient-derived xenograft; P0 and P1, passage 0 and passage 1 (The first generation of PDX is denoted P0, and P0 is subsequently passaged to second generation (P1); Pt., patient; TC, tumor cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars, 20 µm.

Article Snippet: Immortalized human dermal microvascular endothelial cell line (iHDMEC) TIME (ATCC ® CRL-4025TM), and immortalized human umbilical vein endothelial cell line (iHUVEC) HUVEC/TERT 2 (ATCC ® CRL-4053) were purchased from ATCC (Manassas, VA, USA).

Techniques: Marker, Expressing, Derivative Assay

Journal: bioRxiv

Article Title: A Novel Humanized Immune Stroma PDX Cancer Model for Therapeutic Studies

doi: 10.1101/2023.07.03.547206

Figure Lengend Snippet: HIS-PDX were created by co-engraftment of PDX TC, CA-MSC and EC (iHUVEC + iHDMEC). ( a ) IF for alpha smooth muscle actin (αSMA; red), and human-specific mitochondrial antigen (hMitoc; green) or pan-cytokeratin (PanCK; green) to label tumor cells in standard and HIS-PDX. Cell nuclei are counterstained with DAPI (blue). ( b ) Hematoxylin and eosin (H&E) staining for a cell line derived xenografts, standard PDX, humanized immune stroma PDX (HIS-PDX) and matched patient’s primary tumor. ( c ) RNA-seq derived heat map for stromal and immune gene expression in solid tumor derived standard PDXs (StndPDX), HIS-PDX, and patient primary tumor. ( d ) Total gene expression correlation between primary human tumor and HIS-PDX. Scale bars in ( a ), 20 µm; scale bars in ( b ), 200 µm.

Article Snippet: Immortalized human dermal microvascular endothelial cell line (iHDMEC) TIME (ATCC ® CRL-4025TM), and immortalized human umbilical vein endothelial cell line (iHUVEC) HUVEC/TERT 2 (ATCC ® CRL-4053) were purchased from ATCC (Manassas, VA, USA).

Techniques: Staining, Derivative Assay, RNA Sequencing, Gene Expression